Exhaled Gas Analysis by GC-MS, Sampled from Flexible Bronchoscopy, for Finding Lung Cancer Biomarker

Exhaled Gas Analysis by GC-MS, Sampled from Flexible Bronchoscopy, for Finding Lung Cancer Biomarker

Yunghee Lee, Ho Il Yoon

1. Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, Seoul National University Bundang Hospital, Gyeonggi-Do, Republic of Korea

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Abstract:

The probability of lung cancer is increased and the survival is distinct between early and late stage of lung cancer. In this study, we analyze the exhaled gas, collected by bronchoscopy, to find lung cancer biomarker by using thermal desorption of gas chromatograph time of flight mass spectrometer (TD-GC-MS, Markers, Agilent 7890B). Methods Prospective cohort study has been progressed in Seoul National University Bundang Hospital from August, 2019 for 15 patients. Gas sample was collected in cancer affected bronchus (G1), cancer non-affected contralateral bronchus (G2), and room air (G3) in bronchoscopy room. The gas was sampled by the working channel port in bronchoscopy (Olympus, BF-1T260, BF-260, BF-P260F). Results Two patients were excluded and 13 patients’ results were presented. The integration value of each VOCs were calculated by characteristic retention time and 6 high ranks of VOCs in G1 were Dibutyl phthalate (P = 0.546, 3 out of 13), Acetone (P = 0.009, 12 out of 13), Ethanol (P = 0.072, 13 out of 13), Hexane, 2,3,5-trimethyl (P = 0.368, 1 out of 13), 1,4-Pentadiene (P = 0.064, 5 out of 13) and Benzaldehyde 4-methyl (P = 0.417, 9 out of 13). Further gas samples of new patients will be obtained; the aim of enrollment number of patients is 20. Conclusions The qualitative analysis of VOCs in lung cancer was done by TD-GC-MS with sample collection by bronchoscopy to select more targeted gas. There were few VOCs were suggested as lung cancer biomarker.

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