Volatile profiles of MSTO-211H malignant mesothelioma cells

Volatile profiles of MSTO-211H malignant mesothelioma cells

Little LD, Cole L, Allen KE, Haywood-Small SL

Biomolecular Sciences Research Centre, Sheffield Hallam University

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Background - Malignant mesothelioma (MM) is an incurable cancer with a poor survival rate and limited treatment options. Volatile organic compounds (VOCs) in breath have been shown to differentiate MM patients from other groups, with the ultimate intention of providing a non-invasive method of early diagnosis. Headspace analysis was performed on MSTO-211H cell cultures in order to identify the most clinically relevant VOCs released from MM cells. Methods - Solid-phase microextraction (SPME; Supelco, DVB/CAR/PDMS) was performed on MSTO-211H cultures and controls at 24, 48 and 72 hour incubation points. The SPME fibre was transferred to a GC-MS for thermal desorption and VOC analysis. Cell viability was assessed after each time point. Principal component analysis was performed using XCMS online. Results - Cell number increased with incubation but cell viability was maintained. Over 100 VOCs were identified from controls, with some specific changes observed in cell cultures. 2,6-Dimethyl-Octane and 2-butoxyethyl acetate were reduced in cells and 2-butoxy-Ethanol and 2-ethyl-Hexanol were increased compared to controls. PCA score plots showed separation of results after 48 and 72 hours incubation and clustering of experimental repeats. Conclusion - 2-butoxy Ethanol was increased in cell cultures whereas 2-butoxyethyl acetate was decreased - likely to be the result of cellular metabolism through a hydrolysis reaction. The PCA plot showed separation of MSTO-211H and RPMI results, highlighting the potential of VOC analysis to be used in a diagnostic setting. Further method development is required to reduce culture-ware background signals and identify unique VOCs released from MM cells.

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